26.12 Red cell folate (categories)

Red cell folate data available for STOP cohort only.

Within reference ranges (360 to 1400 vs >1400).

26.12.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

PRL (ng/ml)

	Chisq	Df	Pr(>Chisq)
(Intercept)	176.5298310	1	<0.0001	***
rcfolate_gp	0.0020879	1	1.0	ns
GA_samp	75.7290268	1	<0.0001	***

hPL (μg/ml)

	Chisq	Df	Pr(>Chisq)
(Intercept)	41.4726214	1	<0.0001	***
rcfolate_gp	0.2775494	1	0.6	ns
GA_samp	1126.6013558	1	<0.0001	***

GH2 (ng/ml)

	Chisq	Df	Pr(>Chisq)
(Intercept)	420.04231	1	<0.0001	***
rcfolate_gp	6.18705	1	0.01	*
GA_samp	572.93929	1	<0.0001	***

Insulin (uU/ml)

model term	df1	df2	F.ratio	Chisq	p.value
rcfolate_gp	1	Inf	0.418	0.418	0.5	ns
GA_samp	1	Inf	0.761	0.761	0.4	ns

HOMA-IR

model term	df1	df2	F.ratio	Chisq	p.value
rcfolate_gp	1	Inf	0.435	0.435	0.5	ns
GA_samp	1	Inf	0.646	0.646	0.4	ns

Glucose (mmol/L)

model term	df1	df2	F.ratio	Chisq	p.value
rcfolate_gp	1	Inf	4.135	4.135	0.04	*
GA_samp	1	Inf	0.457	0.457	0.5	ns

26.12.2 Model summary

Ratio of Geometric means (95% CI)

PRL (ng/ml)

Var	Effect	P
(Intercept)	14.79 (9.94, 22.00)	<0.0001
rcfolate_gp>1400	1.00 (0.90, 1.11)	0.96
GA_samp	1.15 (1.12, 1.19)	<0.0001

hPL (μg/ml)

Var	Effect	P
(Intercept)	1.98 (1.61, 2.44)	<0.0001
rcfolate_gp>1400	1.02 (0.96, 1.08)	0.60
GA_samp	1.35 (1.33, 1.37)	<0.0001

GH2 (ng/ml)

Var	Effect	P
(Intercept)	0.05 (0.04, 0.06)	<0.0001
rcfolate_gp>1400	0.90 (0.83, 0.98)	0.01
GA_samp	1.35 (1.32, 1.39)	<0.0001

Insulin (uU/ml)

Var	Effect	P
(Intercept)	8.11 (5.00, 13.13)	<0.0001
rcfolate_gp>1400	1.04 (0.92, 1.19)	0.52
GA_samp	1.02 (0.98, 1.06)	0.38

HOMA-IR

Var	Effect	P
(Intercept)	1.71 (1.04, 2.82)	0.03
rcfolate_gp>1400	1.05 (0.91, 1.20)	0.51
GA_samp	1.02 (0.97, 1.06)	0.42

Glucose (mmol/L)

Var	Effect	P
(Intercept)	4.78 (4.35, 5.25)	<0.0001
rcfolate_gp>1400	1.04 (1.00, 1.07)	0.04
GA_samp	1.00 (0.99, 1.00)	0.50

26.12.3 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

26.12.4 Estimated marginal means

Outcome	rcfolate_gp	response	SE	df	lower.CL	upper.CL
PRL (ng/ml)	360 to 1400	74.847	6.442	75.895	63.057	88.843
PRL (ng/ml)	>1400	74.659	6.331	71.843	63.048	88.409
hPL (μg/ml)	360 to 1400	61.851	2.053	74.734	57.894	66.078
hPL (μg/ml)	>1400	62.844	2.025	67.509	58.930	67.018
GH2 (ng/ml)	360 to 1400	1.529	0.063	96.552	1.410	1.658
GH2 (ng/ml)	>1400	1.378	0.054	85.205	1.274	1.490
Insulin (uU/ml)	360 to 1400	10.052	0.468	Inf	9.176	11.013
Insulin (uU/ml)	>1400	10.498	0.504	Inf	9.556	11.533
HOMA-IR	360 to 1400	2.104	0.102	Inf	1.913	2.314
HOMA-IR	>1400	2.204	0.112	Inf	1.996	2.434
Glucose (mmol/L)	360 to 1400	4.648	0.056	Inf	4.540	4.759
Glucose (mmol/L)	>1400	4.818	0.062	Inf	4.698	4.941

26.12.5 Plots

26.12.6 Post-hoc comparisons

Outcome	Contrast	Ratio of Geometric means (95% CI)	P-value
PRL (ng/ml)	>1400 / 360 to 1400	1.00 (0.90, 1.11)	1.0
hPL (μg/ml)	>1400 / 360 to 1400	1.02 (0.96, 1.08)	0.6
GH2 (ng/ml)	>1400 / 360 to 1400	0.90 (0.83, 0.98)	0.01
Insulin (uU/ml)	>1400 / 360 to 1400	1.04 (0.92, 1.19)	0.5
HOMA-IR	>1400 / 360 to 1400	1.05 (0.91, 1.20)	0.5
Glucose (mmol/L)	>1400 / 360 to 1400	1.04 (1.00, 1.07)	0.04