26.12 Red cell folate (categories)

Red cell folate data available for STOP cohort only.


Within reference ranges (360 to 1400 vs >1400).

26.12.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

PRL (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 176.5298310 1 <0.0001 ***
rcfolate_gp 0.0020879 1 1.0 ns
GA_samp 75.7290268 1 <0.0001 ***
hPL (μg/ml)
Chisq Df Pr(>Chisq)
(Intercept) 41.4726214 1 <0.0001 ***
rcfolate_gp 0.2775494 1 0.6 ns
GA_samp 1126.6013558 1 <0.0001 ***
GH2 (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 420.04231 1 <0.0001 ***
rcfolate_gp 6.18705 1 0.01 *
GA_samp 572.93929 1 <0.0001 ***
Insulin (uU/ml)
model term df1 df2 F.ratio Chisq p.value
rcfolate_gp 1 Inf 0.418 0.418 0.5 ns
GA_samp 1 Inf 0.761 0.761 0.4 ns
HOMA-IR
model term df1 df2 F.ratio Chisq p.value
rcfolate_gp 1 Inf 0.435 0.435 0.5 ns
GA_samp 1 Inf 0.646 0.646 0.4 ns
Glucose (mmol/L)
model term df1 df2 F.ratio Chisq p.value
rcfolate_gp 1 Inf 4.135 4.135 0.04 *
GA_samp 1 Inf 0.457 0.457 0.5 ns

26.12.2 Model summary

Ratio of Geometric means (95% CI)

PRL (ng/ml)
Var Effect P
(Intercept) 14.79 (9.94, 22.00) <0.0001
rcfolate_gp>1400 1.00 (0.90, 1.11) 0.96
GA_samp 1.15 (1.12, 1.19) <0.0001
hPL (μg/ml)
Var Effect P
(Intercept) 1.98 (1.61, 2.44) <0.0001
rcfolate_gp>1400 1.02 (0.96, 1.08) 0.60
GA_samp 1.35 (1.33, 1.37) <0.0001
GH2 (ng/ml)
Var Effect P
(Intercept) 0.05 (0.04, 0.06) <0.0001
rcfolate_gp>1400 0.90 (0.83, 0.98) 0.01
GA_samp 1.35 (1.32, 1.39) <0.0001
Insulin (uU/ml)
Var Effect P
(Intercept) 8.11 (5.00, 13.13) <0.0001
rcfolate_gp>1400 1.04 (0.92, 1.19) 0.52
GA_samp 1.02 (0.98, 1.06) 0.38
HOMA-IR
Var Effect P
(Intercept) 1.71 (1.04, 2.82) 0.03
rcfolate_gp>1400 1.05 (0.91, 1.20) 0.51
GA_samp 1.02 (0.97, 1.06) 0.42
Glucose (mmol/L)
Var Effect P
(Intercept) 4.78 (4.35, 5.25) <0.0001
rcfolate_gp>1400 1.04 (1.00, 1.07) 0.04
GA_samp 1.00 (0.99, 1.00) 0.50

26.12.3 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

26.12.4 Estimated marginal means

Outcome rcfolate_gp response SE df lower.CL upper.CL
PRL (ng/ml) 360 to 1400 74.847 6.442 75.895 63.057 88.843
PRL (ng/ml) >1400 74.659 6.331 71.843 63.048 88.409
hPL (μg/ml) 360 to 1400 61.851 2.053 74.734 57.894 66.078
hPL (μg/ml) >1400 62.844 2.025 67.509 58.930 67.018
GH2 (ng/ml) 360 to 1400 1.529 0.063 96.552 1.410 1.658
GH2 (ng/ml) >1400 1.378 0.054 85.205 1.274 1.490
Insulin (uU/ml) 360 to 1400 10.052 0.468 Inf 9.176 11.013
Insulin (uU/ml) >1400 10.498 0.504 Inf 9.556 11.533
HOMA-IR 360 to 1400 2.104 0.102 Inf 1.913 2.314
HOMA-IR >1400 2.204 0.112 Inf 1.996 2.434
Glucose (mmol/L) 360 to 1400 4.648 0.056 Inf 4.540 4.759
Glucose (mmol/L) >1400 4.818 0.062 Inf 4.698 4.941

26.12.5 Plots

26.12.6 Post-hoc comparisons

Outcome Contrast Ratio of Geometric means (95% CI) P-value
PRL (ng/ml) >1400 / 360 to 1400 1.00 (0.90, 1.11) 1.0
hPL (μg/ml) >1400 / 360 to 1400 1.02 (0.96, 1.08) 0.6
GH2 (ng/ml) >1400 / 360 to 1400 0.90 (0.83, 0.98) 0.01
Insulin (uU/ml) >1400 / 360 to 1400 1.04 (0.92, 1.19) 0.5
HOMA-IR >1400 / 360 to 1400 1.05 (0.91, 1.20) 0.5
Glucose (mmol/L) >1400 / 360 to 1400 1.04 (1.00, 1.07) 0.04